Cytometry has become a fundamental technology that enables scientific applications in multiple disciplines including fundamental (Cell Biology), translational (Immunology) and clinical research (Hematology and Oncology).
Why an advanced course in cytometry?
Cytometry has become a fundamental technology that enables scientific applications in multiple disciplines including fundamental (Cell Biology), translational (Immunology) and clinical research (Hematology and Oncology). The last 10 years have witnessed an explosion in the development of fluorescence- and mass-based detection methods that are revolutionizing the field. Recently, genomic cytometry and immunophenotyping on tissue have been added to the arsenal of possibilities within most advanced core facilities. An integral part of the responsibilities of cytometry core facilities is to provide educational support and technical training to facilitate access to cytometry-based methods, in order to ultimately ensure the generation of reproducible quality data and an optimal use of existing resources.
General Learning Objectives
This is a comprehensive course that covers the fundamentals of flow cytometry analysis and sorting, as well as mass cytometry in a lecture format supplemented by practical lab and data analysis sessions. The course is designed to gain in depth knowledge on general technical aspects of the different types of cytometers available in the market and how the different components and their configuration influence data acquisition. Special emphasis is placed on basic concepts of lasers and fluorescence emission, how to properly set up a cytometer for standardized performance, how to optimally prepare cells for a cytometry experiment, the principle of FACS-based cell sorting, and other specialized applications, including data analysis.
Interactive sessions on data analysis will build up upon basic concepts on compensation, data processing and gating and will focus on the analysis of medium-large multicolor panels. Basic understanding of flow cytometry packages is a not prerequisite, but will help a great deal. Our aim is to provide a wide overview of the different modalities of cytometry currently available and all specialized applications that can be used by PhD students in the fields of Immunology, Hematology, and Oncology. The course will conclude with a feedback session with cytometry experts where a selected group of students will present special cases and discuss important aspects of their own PhD projects.
Format
The course consists of four types of sessions; interactive classroom teaching, panel design workgroups, data analysis sessions, and lab demonstrations. For the data analysis sessions, attendants may use their own laptops equipped with a demo version of FCS Express.
Note
Because of restrictions imposed by the COVID-19 crisis, we are not able to offer a classroom-based course. Instead, we have set up an online program split over several focused sessions that covers the most important aspects of the course, with the exception of the live demos.
| Start date: | |
|---|---|
| Time: | |
| End time: | |
| Location: | Online via Zoom |
| Costs: | Free (50 EUR cancellation penalty). Documentation is provided free of charge |
|---|---|
| Register now |
| Organisor |
Juan J. Garcia Vallejo, MD, PhD, MBA |
|---|---|
| Contact | jj.garciavallejo@amsterdamumc.nl |