Over the course of my three-month visit at React4Life in Genoa, Italy, I collaborated with the React4Life team to adapt the MIVO® organ-on-chip system for gastroesophageal adenocarcinoma (GEA). Our work focused on laying the groundwork for an in vitro model that more closely reflects the tumor microenvironment (TME) and could serve as a platform for evaluating innovative immune-targeting therapies.
Piazza de Ferrari in Genoa
Research activities
I began by working with GEA organoids and autologous tumor-infiltrating lymphocytes (TILs) as an accessible starting point before progressing to more complex models. Using the MIVO® device, I examined how fluid flow influenced the organoids and tested different extracellular matrix variants to identify conditions that best supported both tumor cell viability and immune cell function. This work laid the foundation for transferring the technology to Amsterdam UMC, where I will implement the MIVO® system for culturing patient-derived tumor fragments as part of my PhD research line. By directly culturing tumor biopsies or resection specimens in the MIVO® system, I aim to preserve the full native TME in vitro and thus establish a model that better reflects the complexity and heterogeneity seen in GEA patients.
Alongside my laboratory work, I attended workshops and webinars hosted by React4Life, which highlighted different ways the MIVO® system can be applied. These sessions showed how the platform is used to study drug-microbiome interactions, targeted drug delivery and novel immunotherapy strategies and showcased its potential to bridge fundamental research with translational applications.
Experiment using the MIVO® millifluidics device
Life in Genoa
Living in Genoa provided a good balance between research and daily life. The city’s historic streets and rich food culture, especially its famous focaccia, made it an enjoyable place to stay. The nearby beaches in the surrounding towns also offered a pleasant way to spend my free time outside the lab, making the visit both productive and memorable.
Outcomes and impact
The visit provided me with hands-on expertise in organ-on-chip technology and helped establish a stronger collaboration with React4Life. I am currently working with the company to transfer the MIVO® device to Amsterdam UMC, where its implementation will create new opportunities for collaboration within the Cancer Center Amsterdam. Importantly, this exchange has also enabled me to initiate a new research line within my PhD project, and I am grateful to the CCA for their support in making it possible.