NGS
NGS sequencing nowadays is a standard tool in genomic research and diagnostics. The CFG can provide you with a variety of sophisticated platforms for short read to long read sequencing. At the facility we offer high throughput short read sequencing on Illumina and Ion Torrent instruments and Single Molecule (long read) Real Time (SMRT) sequencing on PacBio and Oxford Nanopore instruments.
NGS applications are available for human and other mammal sources as well as for microorganisms and may range from Whole Genome Sequencing to various targeted approaches containing either small or large targets. Sequencing ct/cfDNA or direct RNA is also possible.
Library preparation
We offer library preparation for sequencing of DNA as well as RNA. Several kits are available, from low to normal input, as well as mRNA or total RNA with or without a rRNA depletion. Where possible, we use our automated library preparation liquid handling systems for high throughput generation of libraries but in case of delicate samples, library preparation can also be performed manually. Before and after library preparation the input DNA / RNA as well as the final library will be checked for quality and quantity. Depending on the project needs you can also bring your own prepared DNA or RNA libraries for sequencing.
Questions
Doubting which kit, which sequencer, how to prepare the samples or anything else? The CFG can always be contacted for advice on specific, (non-)standard, library preparations
Photograph by Paulien Kluver